RNAi and CRISPR Gene Editing in Plants
Approaches and Applications
Edited by
Dr. Bushra Tabassum
Release Date: October, 2024
Copyright: 2024
Pages: 54
ISBN: 979-8-9921800-3-9
Book Description
RNAi and CRISPR Gene Editing in Plants - Approaches and Applications examines two groundbreaking genetic modification technologies that are reshaping plant biotechnology and agriculture. This comprehensive work explains both the scientific principles and practical uses of RNA interference (RNAi) and CRISPR-based gene editing systems, giving readers the knowledge they need for both research and implementation.
The book addresses the underlying science and real-world uses of these technologies. It opens with a thorough examination of CRISPR systems, covering their essential components and mechanisms while exploring their integration with chloroplast transformation techniques. This combination creates new possibilities for advancing plant biotechnology across multiple fields, from agriculture to pharmaceutical production.
Key areas covered include:
- Clear explanation of CRISPR mechanisms and components
- In-depth analysis of chloroplast transformation vectors and their functions
- Applications in pharmaceutical production, including vaccine development and antibody bioreactors
- Agricultural stress management techniques, addressing both abiotic (temperature, drought, salinity) and biotic stressors
- RNA interference mechanisms and their applications in pest management
- Practical aspects of genetic transformation techniques, including particle bombardment and PEG-mediated transformation
- Case studies focusing on major crops like potato and maize
This volume serves both researchers in plant biotechnology, agriculture, and related fields.
About Editor
Dr. Bushra Tabassum has completed her Ph.D in the subject of Molecular Biology from CEMB, University of the Punjab under the supervision of Prof. Dr. Idrees Ahmad Nasir and Prof. Dr. Tayyab Husnain. She has served CEMB for 15 years. At SBS, she is working on protection of plants from pathogenic viruses, fungi, and insect pests through the use of RNA Interference technology (siRNA, hpRNA & dsRNA). She has managed seven research projects funded by PARB, HEC, and IFS as Co-PI/PI and has published several research articles in peer-reviewed international journals. Her research interests include: Crop Protection from insect pests, fungi and viruses for which her group make use of all available molecular biology tools including RNAi, Gene Pyramiding, genetic engineering and transgenic strategies to develop a crop product with sustainable yield output.
Research Interests: Plant protection from various biotic (insect pests, viruses, fungi) and abiotic stresses (heat, frost) by employing Molecular Biology techniques like RNAi, transgenic technology, and gene pyramiding.